**ABSTRACT NOT FOR CITATION WITHOUT AUTHOR PERMISSION. The title, authors, and abstract for this completion report are provided below.  For a copy of the full completion report, please contact the author via e-mail at liweim@msu.edu. Questions? Contact the GLFC via email at frp@glfc.org or via telephone at 517-432-2778.**


Technical assistance for chemical aspects of pheromone research


Ugo Bussy and Weiming Li


Department of Fisheries and Wildlife

Michigan State University

East Lansing, MI 48824, United States.


February 2017




This study led to the development, validation and application of several liquid chromatography tandem mass spectrometry methods. Three postdoctoral researchers were trained and participated in the writing of six progress reports and 30 research articles. Many Fisheries and Wildlife graduate students received training in analytical chemistry thought interactions with the postdoctoral trainees.  Expertise was developed to determine various types of chemical entities supporting studies of general biology, endocrinology, evolution and chemical communication in sea lamprey and other lamprey species. Several bioanalytical methods and protocols have been developed and validated (and many published) to measure of bile acids, neuropeptides, neursteroids, neurotransmitters, thyroids and sex steroids hormones in various tissue and environmental matrices.  Through this project, we also provided technical support to numerous projects supported by the Great Lakes Fishery Commission, including projects carried out by federal agencies and academic research laboratories in the United States, Canada and New Zealand.